Data: (++) leaves a mark at 715bp (+-) leaves a mark at both 415bp and 715bp (--) leaves a mark at 415bp
My results were (+-) and my DNA sample was in the 2nd lane from the bottom
Objective: To determine the frequency of the Alu insert among 5th period Bio/Biotech students.
Materials/Reagents: 0.9% Saline solution Student cheek cells 5% Chelex Primer mix Master Mix (nucleotides, DNA polymerase, buffer) TBE buffer Agarose gel, tracking dye DNA stain "Gel Red" Positive control DNA Base Pair Ladder
Procedure: 1. Swish 10mL of saline solution in your mouth for thirty seconds 2. Spit in to the cup 3. Put 1000 ul of cheek cell solution into 1.5 ml microfuge tube 4. Pour supernatant leaving 100uL covering the cell pellet 5. Put 50uL of cell suspension in to tube of chelex 6. Heat in a heating block for 10 minutes 7. Release pressure from tubes 8. Take 50uL of supernatant from tube and place in new microfuge tube
1. Pipet 20uL if master mix, 20uL of primer mix, and 10uL of DNA into new PCR tube 2. Place in thermal cycler
1. 50mL of 1x TAE ab 1g of agarose 2. Heat solution until completely disolved 3. Pour in mold when cool 4. 20 mL